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v ca2 dcn r d systems af1060 polyclonal goat  (R&D Systems)


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    Structured Review

    R&D Systems v ca2 dcn r d systems af1060 polyclonal goat
    V Ca2 Dcn R D Systems Af1060 Polyclonal Goat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+mouse+dcn/Mouse+Decorin+Antibody/pmc12396750__pbio%2E3003355%2Es012-2-55-58
    Average 94 stars, based on 59 article reviews
    v ca2 dcn r d systems af1060 polyclonal goat - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly
    Article Snippet: His 6 -tagged ΔDCN cloned into the pET28a vector with 6 His at the N-terminus was purified with the His-Tag kit (Novagen, Burlington, MA, USA) as described [ ]. .. For Western blotting, lysates were resolved on SDS-PAGE, blotted onto an Immobilon-FL membrane (Millipore, Burlington, MA, USA), blocked with Odyssey blocking buffer (LI-COR), and probed (in PBS/0.05% Triton X-100) with antibodies as follows: goat anti-mouse DCN from R&D Systems (#BAF1060) at 1:1000; rabbit monoclonal anti-Col6A1 (Abcam, Cambridge, MA, USA; ab182744) at 1:1000; rabbit polyclonal anti-Col6A1 (Abcam ab6588) at 1:1000; rabbit polyclonal anti-Co1A1 (Abcam #34710) at 1:1000; Rabbit anti-endotrophin antibody [ ] at 1:1000; Rabbit anti-GFP (Abcam ab290) at 1:1000; and anti-β-Actin (Abcam, Cat. # ab8226, 1:5000). .. The signal was detected using the Odyssey CLx imaging system (LI-COR, Lincoln, NE, USA).

    Membrane:

    Article Title: Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly
    Article Snippet: His 6 -tagged ΔDCN cloned into the pET28a vector with 6 His at the N-terminus was purified with the His-Tag kit (Novagen, Burlington, MA, USA) as described [ ]. .. For Western blotting, lysates were resolved on SDS-PAGE, blotted onto an Immobilon-FL membrane (Millipore, Burlington, MA, USA), blocked with Odyssey blocking buffer (LI-COR), and probed (in PBS/0.05% Triton X-100) with antibodies as follows: goat anti-mouse DCN from R&D Systems (#BAF1060) at 1:1000; rabbit monoclonal anti-Col6A1 (Abcam, Cambridge, MA, USA; ab182744) at 1:1000; rabbit polyclonal anti-Col6A1 (Abcam ab6588) at 1:1000; rabbit polyclonal anti-Co1A1 (Abcam #34710) at 1:1000; Rabbit anti-endotrophin antibody [ ] at 1:1000; Rabbit anti-GFP (Abcam ab290) at 1:1000; and anti-β-Actin (Abcam, Cat. # ab8226, 1:5000). .. The signal was detected using the Odyssey CLx imaging system (LI-COR, Lincoln, NE, USA).

    Blocking Assay:

    Article Title: Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly
    Article Snippet: His 6 -tagged ΔDCN cloned into the pET28a vector with 6 His at the N-terminus was purified with the His-Tag kit (Novagen, Burlington, MA, USA) as described [ ]. .. For Western blotting, lysates were resolved on SDS-PAGE, blotted onto an Immobilon-FL membrane (Millipore, Burlington, MA, USA), blocked with Odyssey blocking buffer (LI-COR), and probed (in PBS/0.05% Triton X-100) with antibodies as follows: goat anti-mouse DCN from R&D Systems (#BAF1060) at 1:1000; rabbit monoclonal anti-Col6A1 (Abcam, Cambridge, MA, USA; ab182744) at 1:1000; rabbit polyclonal anti-Col6A1 (Abcam ab6588) at 1:1000; rabbit polyclonal anti-Co1A1 (Abcam #34710) at 1:1000; Rabbit anti-endotrophin antibody [ ] at 1:1000; Rabbit anti-GFP (Abcam ab290) at 1:1000; and anti-β-Actin (Abcam, Cat. # ab8226, 1:5000). .. The signal was detected using the Odyssey CLx imaging system (LI-COR, Lincoln, NE, USA).



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    Fig. 4 Topical application of VTI-1002 gel promotes tissue remodeling in diabetic burn wounds. Representative images of a FBN (fibronectin), b <t>DCN</t> (decorin) staining, and quantification of a FBN, b DCN staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. c Representative images of Picrosirius Red staining and quantification of staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. d Representative second- harmonic generation (SHG) imaging and quantification of SHG signal intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 50 µm. e Comparison of type <t>I/III</t> <t>collagen</t> ratio in the wounds at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). f Comparison of post-wound skin tensile strength at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). Results are expressed as median and min/max value, NS = not significant, *P < 0.05, **P < 0.01 by Student's t test
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    Fig. 4 Topical application of VTI-1002 gel promotes tissue remodeling in diabetic burn wounds. Representative images of a FBN (fibronectin), b <t>DCN</t> (decorin) staining, and quantification of a FBN, b DCN staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. c Representative images of Picrosirius Red staining and quantification of staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. d Representative second- harmonic generation (SHG) imaging and quantification of SHG signal intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 50 µm. e Comparison of type <t>I/III</t> <t>collagen</t> ratio in the wounds at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). f Comparison of post-wound skin tensile strength at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). Results are expressed as median and min/max value, NS = not significant, *P < 0.05, **P < 0.01 by Student's t test
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    Image Search Results


    Fig. 4 Topical application of VTI-1002 gel promotes tissue remodeling in diabetic burn wounds. Representative images of a FBN (fibronectin), b DCN (decorin) staining, and quantification of a FBN, b DCN staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. c Representative images of Picrosirius Red staining and quantification of staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. d Representative second- harmonic generation (SHG) imaging and quantification of SHG signal intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 50 µm. e Comparison of type I/III collagen ratio in the wounds at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). f Comparison of post-wound skin tensile strength at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). Results are expressed as median and min/max value, NS = not significant, *P < 0.05, **P < 0.01 by Student's t test

    Journal: Experimental & molecular medicine

    Article Title: Topical small molecule granzyme B inhibitor improves remodeling in a murine model of impaired burn wound healing.

    doi: 10.1038/s12276-018-0095-0

    Figure Lengend Snippet: Fig. 4 Topical application of VTI-1002 gel promotes tissue remodeling in diabetic burn wounds. Representative images of a FBN (fibronectin), b DCN (decorin) staining, and quantification of a FBN, b DCN staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. c Representative images of Picrosirius Red staining and quantification of staining intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 100 µm. d Representative second- harmonic generation (SHG) imaging and quantification of SHG signal intensity in the wounds at day 30 from vehicle control group and VTI-1002 treatment group (n ≥5 per group). Scale bar = 50 µm. e Comparison of type I/III collagen ratio in the wounds at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). f Comparison of post-wound skin tensile strength at day 30 between vehicle control group and VTI-1002 treatment group (n ≥5 per group). Results are expressed as median and min/max value, NS = not significant, *P < 0.05, **P < 0.01 by Student's t test

    Article Snippet: Immunohistochemistry was performed using rat antimouse CD45 antibody (BD Biosciences), rabbit polyclonal CD68 antibody (Abcam), rabbit anti-mouse vimentin antibody (Cell Signaling), rabbit anti-mouse α-smooth muscle actin antibody (Abcam), anti-pan keratin antibody (Abcam), rabbit anti-collagen I antibody (Abcam), rabbit anti-collagen III antibody (Abcam), rabbit anti-FBN antibody (Abcam), and goat anti-mouse DCN antibody (R&D Systems, Minneapolis, MN, USA) as described previously10.

    Techniques: Staining, Control, Imaging, Comparison