v ca2 dcn r d systems af1060 polyclonal goat (R&D Systems)
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V Ca2 Dcn R D Systems Af1060 Polyclonal Goat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+mouse+dcn/Mouse+Decorin+Antibody/pmc12396750__pbio%2E3003355%2Es012-2-55-58
Average 94 stars, based on 59 article reviews
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Western Blot:Article Title: Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly Article Snippet: His 6 -tagged ΔDCN cloned into the pET28a vector with 6 His at the N-terminus was purified with the His-Tag kit (Novagen, Burlington, MA, USA) as described [ ]. .. For Western blotting, lysates were resolved on SDS-PAGE, blotted onto an Immobilon-FL membrane (Millipore, Burlington, MA, USA), blocked with Odyssey blocking buffer (LI-COR), and probed (in PBS/0.05% Triton X-100) with antibodies as follows: Membrane:Article Title: Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly Article Snippet: His 6 -tagged ΔDCN cloned into the pET28a vector with 6 His at the N-terminus was purified with the His-Tag kit (Novagen, Burlington, MA, USA) as described [ ]. .. For Western blotting, lysates were resolved on SDS-PAGE, blotted onto an Immobilon-FL membrane (Millipore, Burlington, MA, USA), blocked with Odyssey blocking buffer (LI-COR), and probed (in PBS/0.05% Triton X-100) with antibodies as follows: Blocking Assay:Article Title: Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly Article Snippet: His 6 -tagged ΔDCN cloned into the pET28a vector with 6 His at the N-terminus was purified with the His-Tag kit (Novagen, Burlington, MA, USA) as described [ ]. .. For Western blotting, lysates were resolved on SDS-PAGE, blotted onto an Immobilon-FL membrane (Millipore, Burlington, MA, USA), blocked with Odyssey blocking buffer (LI-COR), and probed (in PBS/0.05% Triton X-100) with antibodies as follows: |
